Reperfusion promotes mitochondrial biogenesis following focal cerebral ischemia in rats.

ABSTRACT:

Background and purpose

Reperfusion after transient cerebral ischemia causes severe damage to mitochondria; however, little is known regarding the continuous change in mitochondrial biogenesis during reperfusion. Mitochondrial biogenesis causes an increase in the individual mitochondrial mass of neurons and maintains their aerobic set-point in the face of declining function. The aim of this study was to examine mitochondrial biogenesis in the cortex during reperfusion following focal cerebral ischemia.

Methods

Male Wistar rats were subjected to transient focal cerebral ischemia. The relative amount of cortical mitochondrial DNA was analyzed using quantitative real-time PCR at 0 h, 24 h, 72 h, and 7 d after reperfusion. Three critical transcriptional regulators of mitochondria

SUBMITTER: Xie Y 

PROVIDER: S-EPMC3965405 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature