Design of optimally constructed metabolic networks of minimal functionality.

ABSTRACT:

Background

Metabolic engineering aims to design microorganisms that will generate a product of interest at high yield. Thus, a variety of in silico modeling strategies has been applied successfully, including the concepts of elementary flux modes (EFMs) and constrained minimal cut sets (cMCSs). The EFMs (minimal, steady state pathways through the system) can be calculated given a metabolic model. cMCSs are sets of reaction deletions in such a network that will allow desired pathways to survive and disable undesired ones (e.g., those with low product secretion or low growth rates). Grouping the modes into desired and undesired categories had to be done manually until now.

Results

Although the optimal solution for a given set of pathways will always be found with the currently

SUBMITTER: Ruckerbauer DE 

PROVIDER: S-EPMC3965433 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature