Cloning and characterization of a wheat homologue of apurinic/apyrimidinic endonuclease Ape1L.

ABSTRACT:

Background

Apurinic/apyrimidinic (AP) endonucleases are key DNA repair enzymes involved in the base excision repair (BER) pathway. In BER, an AP endonuclease cleaves DNA at AP sites and 3'-blocking moieties generated by DNA glycosylases and/or oxidative damage. A Triticum aestivum cDNA encoding for a putative homologue of ExoIII family AP endonucleases which includes E. coli Xth, human APE1 and Arabidopsis thaliana AtApe1L has been isolated and its protein product purified and characterized.

Methodology/principal findings

We report that the putative wheat AP endonuclease, referred here as TaApe1L, contains AP endonuclease, 3'-repair phosphodiesterase, 3'-phosphatase and 3' → 5' exonuclease activities. Surprisingly, in contrast to bacterial and human AP endonucleases, additio

SUBMITTER: Joldybayeva B 

PROVIDER: S-EPMC3965494 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature