Association of proinflammatory cytokines and islet resident leucocytes with islet dysfunction in type 2 diabetes.

ABSTRACT:

Aims/hypothesis

Chronic inflammation in type 2 diabetes is proposed to affect islets as well as insulin target organs. However, the nature of islet inflammation and its effects on islet function in type 2 diabetes remain unclear. Moreover, the immune cell profiles of human islets in healthy and type 2 diabetic conditions are undefined. We aimed to investigate the correlation between proinflammatory cytokine expression, islet leucocyte composition and insulin secretion in type 2 diabetic human islets.

Methods

Human islets from organ donors with or without type 2 diabetes were studied. First and second phases of glucose-stimulated insulin secretion were determined by perifusion. The expression of inflammatory markers was obtained by quantitative PCR. Immune cells within human

SUBMITTER: Butcher MJ 

PROVIDER: S-EPMC3966210 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature