Genome-wide probing of RNA structure reveals active unfolding of mRNA structures in vivo.

ABSTRACT: RNA has a dual role as an informational molecule and a direct effector of biological tasks. The latter function is enabled by RNA's ability to adopt complex secondary and tertiary folds and thus has motivated extensive computational and experimental efforts for determining RNA structures. Existing approaches for evaluating RNA structure have been largely limited to in vitro systems, yet the thermodynamic forces which drive RNA folding in vitro may not be sufficient to predict stable RNA structures in vivo. Indeed, the presence of RNA-binding proteins and ATP-dependent helicases can influence which structures are present inside cells. Here we present an approach for globally monitoring RNA structure in native conditions in vivo with single-nucleotide precision. This method is based on in vi

SUBMITTER: Rouskin S 

PROVIDER: S-EPMC3966492 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature