Exercise prevents weight gain and alters the gut microbiota in a mouse model of high fat diet-induced obesity.

ABSTRACT:

Background

Diet-induced obesity (DIO) is a significant health concern which has been linked to structural and functional changes in the gut microbiota. Exercise (Ex) is effective in preventing obesity, but whether Ex alters the gut microbiota during development with high fat (HF) feeding is unknown.

Objective

Determine the effects of voluntary Ex on the gastrointestinal microbiota in LF-fed mice and in HF-DIO.

Methods

Male C57BL/6 littermates (5 weeks) were distributed equally into 4 groups: low fat (LF) sedentary (Sed) LF/Sed, LF/Ex, HF/Sed and HF/Ex. Mice were individually housed and LF/Ex and HF/Ex cages were equipped with a wheel and odometer to record Ex. Fecal samples were collected at baseline, 6 weeks and 12 weeks and used for bacterial DNA isolation. DNA was

SUBMITTER: Evans CC 

PROVIDER: S-EPMC3966766 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature