High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells.

ABSTRACT: Disease caused by dengue virus is a global health concern with up to 390 million individuals infected annually worldwide. There are no vaccines or antiviral compounds available to either prevent or treat dengue disease which may be fatal. To increase our understanding of the interaction of dengue virus with the host cell, we analyzed changes in the proteome of human A549 cells in response to dengue virus type 2 infection using stable isotope labelling in cell culture (SILAC) in combination with high-throughput mass spectrometry (MS). Mock and infected A549 cells were fractionated into nuclear and cytoplasmic extracts before analysis to identify proteins that redistribute between cellular compartments during infection and reduce the complexity of the analysis. We identified and quantified 3

SUBMITTER: Chiu HC 

PROVIDER: S-EPMC3966871 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature