The translocation selectivity of the kinesins that mediate neuronal organelle transport.

ABSTRACT: Polarized kinesin-driven transport is crucial for development and maintenance of neuronal polarity. Kinesins are thought to recognize biochemical differences between axonal and dendritic microtubules in order to deliver their cargoes to the appropriate domain. To identify kinesins that mediate polarized transport, we prepared constitutively active versions of all the kinesins implicated in vesicle transport and expressed them in cultured hippocampal neurons. Seven kinesins translocated preferentially to axons and five translocated into both axons and dendrites. None translocated selectively to dendrites. Highly homologous members of the same subfamily displayed distinctly different translocation preferences and were differentially regulated during development. By expressing chimeric kinesi

SUBMITTER: Huang CF 

PROVIDER: S-EPMC3967410 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature