Comprehensive assessment and standardization of solid phase multiplex-bead arrays for the detection of antibodies to HLA.

ABSTRACT: Solid phase multiplex-bead arrays for the detection and characterization of HLA antibodies provide increased sensitivity and specificity compared to conventional lymphocyte-based assays. Assay variability due to inconsistencies in commercial kits and differences in standard operating procedures (SOP) hamper comparison of results between laboratories. The Clinical Trials in Organ Transplantation Antibody Core Laboratories investigated sources of assay variation and determined if reproducibility improved through utilization of SOP, common reagents and normalization algorithms. Ten commercial kits from two manufacturers were assessed in each of seven laboratories using 20 HLA reference sera. Implementation of a standardized (vs. a nonstandardized) operating procedure greatly reduced MFI varia

SUBMITTER: Reed EF 

PROVIDER: S-EPMC3967448 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature