Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy.

ABSTRACT: To enhance silencing and avoid off-target effects, siRNAs are often designed with an intentional bias to ensure that the end of the siRNA that contains the guide strand 5' end is less stably hybridized relative to the end containing the passenger strand 5' end. One means by which this is accomplished is to introduce a terminal mismatch, typically by changing the passenger strand sequence to impair its hybridization with the guide strand 5' end. However, there are conflicting reports about the influence of terminal mismatches on the silencing efficacy of siRNAs. Here, the silencing efficiency of siRNAs with a terminal mismatch generated either by altering the guide strand (at the 5' end, nucleotide 1) or the passenger strand (nucleotide 19 from the 5' end) was examined. Subsequently, we stu

SUBMITTER: Kini HK 

PROVIDER: S-EPMC3967519 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature