Transforming growth factor β signaling controls activities of human intestinal CD8(+)T suppressor cells.

ABSTRACT:

Background & aims

In healthy individuals, interactions between intestinal epithelial cells and lamina propria lymphocytes give rise to a population of CD8(+) T cells with suppressor functions (Ts cells). Disruption of Ts cell activities can lead to mucosal inflammation. We investigated what factors were required for expansion of the Ts cell population or loss of their activity in patients with Crohn's disease (CD).

Methods

We developed a method to generate Ts cell lines from freshly isolated lamina propria lymphocytes from patients with ulcerative colitis (UC), patients with CD, or healthy individuals (controls). Cells were stimulated with a monoclonal antibody against CD3, interleukin (IL)-7, and IL-15. After 14 days in culture, CD8(+)T cells were purified and cultured with

SUBMITTER: Rabinowitz KM 

PROVIDER: S-EPMC3967796 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature