ER sheet persistence is coupled to myosin 1c-regulated dynamic actin filament arrays.

ABSTRACT: The endoplasmic reticulum (ER) comprises a dynamic three-dimensional (3D) network with diverse structural and functional domains. Proper ER operation requires an intricate balance within and between dynamics, morphology, and functions, but how these processes are coupled in cells has been unclear. Using live-cell imaging and 3D electron microscopy, we identify a specific subset of actin filaments localizing to polygons defined by ER sheets and tubules and describe a role for these actin arrays in ER sheet persistence and, thereby, in maintenance of the characteristic network architecture by showing that actin depolymerization leads to increased sheet fluctuation and transformations and results in small and less abundant sheet remnants and a defective ER network distribution. Furthermore, w

SUBMITTER: Joensuu M 

PROVIDER: S-EPMC3967974 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature