Identification of internal reference genes for gene expression normalization between the two sexes in dioecious white Campion.

ABSTRACT: Quantitative real time (qRT)-PCR is a precise and efficient method for studying gene expression changes between two states of interest, and is frequently used for validating interesting gene expression patterns in candidate genes initially identified in genome-wide expression analyses, such as RNA-seq experiments. For an adequate normalisation of qRT-PCR data, it is essential to have reference genes available whose expression intensities are constant among the different states of interest. In this study we present and validate a catalogue of traditional and newly identified reference genes that were selected from RNA-seq data from multiple individuals from the dioecious plant Silene latifolia with the aim of studying gene expression differences between the two sexes in both reproductive an

SUBMITTER: Zemp N 

PROVIDER: S-EPMC3968030 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature