Digital multiplexed mRNA analysis of functionally important genes in single human oocytes and correlation of changes in transcript levels with oocyte protein expression.

ABSTRACT:

Objective

To investigate functionally important transcripts in single human oocytes with the use of NanoString technology and determine whether observed differences are biologically meaningful.

Design

Analysis of human oocytes with the use of NanoString and immunoblotting.

Setting

University-affiliated reproductive medicine unit.

Patients

Women undergoing in vitro fertilization.

Intervention

Human oocytes were analyzed with the use of NanoString or immunoblotting.

Main outcome measures

The abundance of transcripts for ten functionally important genes-AURKA, AURKC, BUB1, BUB1B (encoding BubR1), CDK1, CHEK1, FYN, MOS, MAP2K1, and WEE2-and six functionally dispensable genes were analyzed with the use of NanoString. BubR1 protein levels in oocytes fro

SUBMITTER: Riris S 

PROVIDER: S-EPMC3969224 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature