EB1 accelerates two conformational transitions important for microtubule maturation and dynamics.

ABSTRACT:

Background

The dynamic properties of microtubules depend on complex nanoscale structural rearrangements in their end regions. Members of the EB1 and XMAP215 protein families interact autonomously with microtubule ends. EB1 recruits several other proteins to growing microtubule ends and has seemingly antagonistic effects on microtubule dynamics: it induces catastrophes, and it increases growth velocity, as does the polymerase XMAP215.

Results

Using a combination of in vitro reconstitution, time-lapse fluorescence microscopy, and subpixel-precision image analysis and convolved model fitting, we have studied the effects of EB1 on conformational transitions in growing microtubule ends and on the time course of catastrophes. EB1 density distributions at growing microtubule ends r

SUBMITTER: Maurer SP 

PROVIDER: S-EPMC3969257 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature