High-throughput sequencing and de novo assembly of Brassica oleracea var. Capitata L. for transcriptome analysis.

ABSTRACT:

Background

The cabbage, Brassica oleracea var. capitata L., has a distinguishable phenotype within the genus Brassica. Despite the economic and genetic importance of cabbage, there is little genomic data for cabbage, and most studies of Brassica are focused on other species or other B. oleracea subspecies. The lack of genomic data for cabbage, a non-model organism, hinders research on its molecular biology. Hence, the construction of reliable transcriptomic data based on high-throughput sequencing technologies is needed to enhance our understanding of cabbage and provide genomic information for future work.

Methodology/principal findings

We constructed cDNAs from total RNA isolated from the roots, leaves, flowers, seedlings, and calcium-limited seedling tissues of two cabbag

SUBMITTER: Kim HA 

PROVIDER: S-EPMC3969326 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature