Interferon-γ induces senescence in normal human melanocytes.

ABSTRACT:

Background

Interferon-γ (IFN-γ) plays an important role in the proceedings of vitiligo through recruiting lymphocytes to the lesional skin. However, the potential effects of IFN-γ on skin melanocytes and the subsequent contribution to the vitiligo pathogenesis are still unclear.

Objective

To investigate the effects of IFN-γ on viability and cellular functions of melanocytes.

Methods

Primary human melanocytes were treated with IFN-γ. Cell viability, apoptosis, cell cycle melanin content and intracellular reactive oxygen species (ROS) level were measured. mRNA expression was examined by real-time PCR. The release of interleukin 6 (IL-6) and heat shock protein 70 (HSP-70) was monitored by ELISA. β-galactosidase staining was utilized to evaluate melanocyte senescence.

SUBMITTER: Wang S 

PROVIDER: S-EPMC3969336 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature