A high-throughput colorimetric screening assay for terpene synthase activity based on substrate consumption.

ABSTRACT: Terpene synthases catalyze the formation of a variety of terpene chemical structures. Systematic mutagenesis studies have been effective in providing insights into the characteristic and complex mechanisms of C-C bond formations and in exploring the enzymatic potential for inventing new chemical structures. In addition, there is growing demand to increase terpene synthase activity in heterologous hosts, given the maturation of metabolic engineering and host breeding for terpenoid synthesis. We have developed a simple screening method for the cellular activities of terpene synthases by scoring their substrate consumption based on the color loss of the cell harboring carotenoid pathways. We demonstrate that this method can be used to detect activities of various terpene synthase or prenyltra

SUBMITTER: Furubayashi M 

PROVIDER: S-EPMC3969365 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature