Analysis of compaction initiation in human embryos by using time-lapse cinematography.

ABSTRACT:

Purpose

To analyze the initiation of compaction in human embryos in vitro by using time-lapse cinematography (TLC), with the goal of determining the precise timing of compaction and clarifying the morphological changes underlying the compaction process.

Methods

One hundred and fifteen embryos donated by couples with no further need for embryo-transfer were used in this study. Donated embryos were thawed and processed, and then their morphological behavior during the initiation of compaction was dynamically observed via time-lapse cinematography (TLC) for 5 days.

Results

Although the initiation of compaction occurred throughout the period from the 4-cell to 16-cell stage, 99 (86.1 %) embryos initiated compaction at the 8-cell stage or later, with initiation at the 8-ce

SUBMITTER: Iwata K 

PROVIDER: S-EPMC3969466 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature