Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms.

ABSTRACT: Despite advances in monitoring spatiotemporal expression patterns of genes and proteins with fluorescent probes, direct detection of metabolites and small molecules remains challenging. A technique for spatially resolved detection of small molecules would benefit the study of redox-active metabolites that are produced by microbial biofilms and can affect their development. Here we present an integrated circuit-based electrochemical sensing platform featuring an array of working electrodes and parallel potentiostat channels. 'Images' over a 3.25 × 0.9 mm(2) area can be captured with a diffusion-limited spatial resolution of 750 μm. We demonstrate that square wave voltammetry can be used to detect, identify and quantify (for concentrations as low as 2.6 μM) four distinct redox-active metabol

SUBMITTER: Bellin DL 

PROVIDER: S-EPMC3969851 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature