Genomic and functional characteristics of human cytomegalovirus revealed by next-generation sequencing.

ABSTRACT: The complete genome of human cytomegalovirus (HCMV) was elucidated almost 25 years ago using a traditional cloning and Sanger sequencing approach. Analysis of the genetic content of additional laboratory and clinical isolates has lead to a better, albeit still incomplete, definition of the coding potential and diversity of wild-type HCMV strains. The introduction of a new generation of massively parallel sequencing technologies, collectively called next-generation sequencing, has profoundly increased the throughput and resolution of the genomics field. These increased possibilities are already leading to a better understanding of the circulating diversity of HCMV clinical isolates. The higher resolution of next-generation sequencing provides new opportunities in the study of intrahost vira

SUBMITTER: Sijmons S 

PROVIDER: S-EPMC3970138 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature