The brain tumor window model: a combined cranial window and implanted glioma model for evaluating intraoperative contrast agents.

ABSTRACT:

Objective

Optical contrast agents for brain tumor delineation have been previously evaluated in ex vivo specimens from animals with implanted gliomas and may not reflect the true visual parameters encountered during surgery. This study describes a novel model system designed to evaluate optical contrast agents for tumor delineation in vivo.

Methods

Biparietal craniectomies were performed on 8-week-old Sprague-Dawley rats. 9L glioma cells were injected intraparenchymally. A cover slip was bonded to the cranial defect with cyanoacrylate glue. When the tumor radius reached 1 mm, Coomassie Blue was administered intravenously while the appearance of the cortical surface was recorded. Computerized image analysis of the red/green/blue color components was used to quantify visible d

SUBMITTER: Orringer DA 

PROVIDER: S-EPMC3970731 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature