PCBP2 siRNA reverses the alcohol-induced pro-fibrogenic effects in hepatic stellate cells.

ABSTRACT:

Purpose

Type I collagen accumulates during liver fibrosis primarily because α-complex protein-2 (αCP(2)), encoded by the poly(rC) binding protein 2 (PCBP2) gene, binds to the 3' end of the collagen mRNA and increases its half-life. This study aimed to reverse the pro-fibrogenic effect of alcohol on hepatic stellate cells (HSCs) by silencing the PCBP2 gene with siRNA.

Methods

The silencing effects of a series of predesigned PCBP2 siRNAs were evaluated in the rat hepatic stellate cell line, HSC-T6. The pro-fibrogenic effects of alcohol on the expression levels of PCBP2 and type-I collagen were examined by several methods. The effect of PCBP2 siRNA on the stability of type I collagen α1(I) mRNA was investigated by an in vitro mRNA decay assay.

Results

We identified one p

SUBMITTER: Shukla RS 

PROVIDER: S-EPMC3970775 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature