Prohibitin viral gene transfer protects hippocampal CA1 neurons from ischemia and ameliorates postischemic hippocampal dysfunction.

ABSTRACT:

Background and purpose

Prohibitin is a multi-functional protein involved in numerous cellular activities. Prohibitin overexpression protects neurons from injury in vitro, but it is unclear whether prohibitin can protect selectively vulnerable hippocampal CA1 neurons in a clinically relevant injury model in vivo and, if so, whether the salvaged neurons remain functional.

Methods

A mouse model of transient forebrain ischemia that mimics the brain damage produced by cardiac arrest in humans was used to test whether prohibitin expression protects CA1 neurons from injury. Prohibitin-expressing viral vector was microinjected in mouse hippocampus to upregulate prohibitin.

Results

Prohibitin overexpression protected CA1 neurons from transient forebrain ischemia. The protectio

SUBMITTER: Kurinami H 

PROVIDER: S-EPMC3971834 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature