High-throughput screening platform for engineered nanoparticle-mediated genotoxicity using CometChip technology.

ABSTRACT: The likelihood of intentional and unintentional engineered nanoparticle (ENP) exposure has dramatically increased due to the use of nanoenabled products. Indeed, ENPs have been incorporated in many useful products and have enhanced our way of life. However, there are many unanswered questions about the consequences of nanoparticle exposures, in particular, with regard to their potential to damage the genome and thus potentially promote cancer. In this study, we present a high-throughput screening assay based upon the recently developed CometChip technology, which enables evaluation of single-stranded DNA breaks, abasic sites, and alkali-sensitive sites in cells exposed to ENPs. The strategic microfabricated, 96-well design and automated processing improves efficiency, reduces processing ti

SUBMITTER: Watson C 

PROVIDER: S-EPMC3971959 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature