Developmental patterns of sleep slow wave activity and synaptic density in adolescent mice.

ABSTRACT:

Study objective

In humans sleep slow wave activity (SWA) declines during adolescence. It has been suggested that this decline reflects the elimination of cortical synapses, but this hypothesis has never been tested directly.

Design

We focused on mouse frontal cortex and collected data from early adolescence (∼postnatal day 20, P20) to adulthood (P60) of (1) SWA; (2) expression of synapsin I, a presynaptic marker; and (3) number of dendritic spines in layers I-II.

Setting

Basic sleep research laboratory.

Patients or participants

YFP-line H mice (n = 70; P15-87, all males) and GFP-line S mice (n = 14; P17-60, 8 females) were used for EEG recording. Forty-five YFP mice (P19-119, 12 females) and 42 GFP-S mice (P20-60, 14 females) were used for in vivo 2-photon imag

SUBMITTER: de Vivo L 

PROVIDER: S-EPMC3972420 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature