Comparative ribosome profiling reveals extensive translational complexity in different Trypanosoma brucei life cycle stages.

ABSTRACT: While gene expression is a fundamental and tightly controlled cellular process that is regulated at multiple steps, the exact contribution of each step remains unknown in any organism. The absence of transcription initiation regulation for RNA polymerase II in the protozoan parasite Trypanosoma brucei greatly simplifies the task of elucidating the contribution of translation to global gene expression. Therefore, we have sequenced ribosome-protected mRNA fragments in T. brucei, permitting the genome-wide analysis of RNA translation and translational efficiency. We find that the latter varies greatly between life cycle stages of the parasite and ∼100-fold between genes, thus contributing to gene expression to a similar extent as RNA stability. The ability to map ribosome positions at sub-cod

SUBMITTER: Vasquez JJ 

PROVIDER: S-EPMC3973304 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature