Sugar-modified G-quadruplexes: effects of LNA-, 2'F-RNA- and 2'F-ANA-guanosine chemistries on G-quadruplex structure and stability.

ABSTRACT: G-quadruplex-forming oligonucleotides containing modified nucleotide chemistries have demonstrated promising pharmaceutical potential. In this work, we systematically investigate the effects of sugar-modified guanosines on the structure and stability of a (4+0) parallel and a (3+1) hybrid G-quadruplex using over 60 modified sequences containing a single-position substitution of 2'-O-4'-C-methylene-guanosine ((LNA)G), 2'-deoxy-2'-fluoro-riboguanosine ((F)G) or 2'-deoxy-2'-fluoro-arabinoguanosine ((FANA)G). Our results are summarized in two parts: (I) Generally, (LNA)G substitutions into 'anti' position guanines within a guanine-tetrad lead to a more stable G-quadruplex, while substitutions into 'syn' positions disrupt the native G-quadruplex conformation. However, some interesting exception

SUBMITTER: Li Z 

PROVIDER: S-EPMC3973314 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature