A dynamic alternative splicing program regulates gene expression during terminal erythropoiesis.

ABSTRACT: Alternative pre-messenger RNA splicing remodels the human transcriptome in a spatiotemporal manner during normal development and differentiation. Here we explored the landscape of transcript diversity in the erythroid lineage by RNA-seq analysis of five highly purified populations of morphologically distinct human erythroblasts, representing the last four cell divisions before enucleation. In this unique differentiation system, we found evidence of an extensive and dynamic alternative splicing program encompassing genes with many diverse functions. Alternative splicing was particularly enriched in genes controlling cell cycle, organelle organization, chromatin function and RNA processing. Many alternative exons exhibited differentiation-associated switches in splicing efficiency, mostly in

SUBMITTER: Pimentel H 

PROVIDER: S-EPMC3973340 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature