Kruppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation.

ABSTRACT: The role of different lineage specific transcription factors in directing hematopoietic cell fate towards myeloid lineage is well established but the status of epigenetic modifications has not been defined during this important developmental process. We used non proliferating, PU.1 inducible myeloid progenitor cells and differentiating bone marrow derived macrophages to study the PU.1 dependent KLF4 transcriptional regulation and its promoter demethylation during monocyte/macrophage differentiation. Expression of KLF4 was regulated by active demethylation of its promoter and PU.1 specifically bound to KLF4 promoter oligo harboring the PU.1 consensus sequence. Methylation specific quantitative PCR and Bisulfite sequencing indicated demethylation of CpG residues most proximal to the transcri

SUBMITTER: Karpurapu M 

PROVIDER: S-EPMC3973678 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature