Identification of differentially expressed proteins from Leishmania amazonensis associated with the loss of virulence of the parasites.

ABSTRACT:

Background

The present study analyzed whether or not the in vitro cultivation for long periods of time of pre-isolated Leishmania amazonensis from lesions of chronically infected BALB/c mice was able to interfere in the parasites' infectivity using in vivo and in vitro experiments. In addition, the proteins that presented a significant decrease or increase in their protein expression content were identified applying a proteomic approach.

Methodology/principal findings

Parasites were cultured in vitro for 150 days. Aliquots were collected on the day 0 of culture (R0), as well as after ten (R10; 50 days of culture), twenty (R20; 100 days of culture), and thirty (R30; 150 days of culture) passages, and were used to analyze the parasites' in vitro and in vivo infectivity, as wel

SUBMITTER: Magalhaes RD 

PROVIDER: S-EPMC3974679 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature