Identification of candidate reference genes in perennial ryegrass for quantitative RT-PCR under various abiotic stress conditions.

ABSTRACT:

Background

Quantitative real-time reverse-transcriptase PCR (qRT-PCR) is an important technique for analyzing differences in gene expression due to its sensitivity, accuracy and specificity. However, the stability of the expression of reference genes is necessary to ensure accurate qRT-PCR assessment of expression in genes of interest. Perennial ryegrass (Lolium perenne L.) is important forage and turf grass species in temperate regions, but the expression stability of its reference genes under various stresses has not been well-studied.

Methodology/principal findings

In this study, 11 candidate reference genes were evaluated for use as controls in qRT-PCR to quantify gene expression in perennial ryegrass under drought, high salinity, heat, waterlogging, and ABA (abscisic ac

SUBMITTER: Huang L 

PROVIDER: S-EPMC3974806 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature