Enhanced identification of zero-length chemical cross-links using label-free quantitation and high-resolution fragment ion spectra.

ABSTRACT: Chemical cross-linking coupled to mass spectrometry provides structural information that is useful for probing protein conformations and providing experimental support for molecular models. "Zero-length" cross-links have greater value for these applications than longer cross-links because they provide more stringent distance constraints. However, this method is less commonly utilized because it cannot take advantage of isotopic labels, MS-labile bonds, or enrichment tags to facilitate identification. In this study, we combined label-free precursor ion quantitation and targeted tandem mass spectrometry with a new software tool, Zero-length Cross-link Miner (ZXMiner), to form a multitiered analysis strategy. A major, critical objective was to simultaneously achieve very high accuracy with es

SUBMITTER: Sriswasdi S 

PROVIDER: S-EPMC3975668 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature