Simultaneous detection of hepatitis C virus and interferon stimulated gene expression in infected human liver.

ABSTRACT:

Unlabelled

Approximately 50% of patients with chronic hepatitis C (CHC) have ongoing expression of interferon stimulated genes (ISGs) in the liver. It is unclear why this endogenous antiviral response is inefficient in eradicating the infection. Several viral escape strategies have been identified in vitro, including inhibition of interferon (IFN) induction and ISG messenger RNA (mRNA) translation. The in vivo relevance of these mechanisms is unknown, because reliable methods to identify hepatitis C virus (HCV)-infected cells in human liver are lacking. We developed a highly sensitive in situ hybridization (ISH) system capable of HCV RNA and ISG mRNA detection in human liver biopsies and applied it to study the interaction of HCV with the endogenous IFN system. We simultaneously mo

SUBMITTER: Wieland S 

PROVIDER: S-EPMC3975814 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature