Down-regulation of integrin β1 and focal adhesion kinase in renal glomeruli under various hemodynamic conditions.

ABSTRACT: Given that integrin β1 is an important component of the connection to maintain glomerular structural integrity, by binding with multiple extracellular matrix proteins and mediating intracellular signaling. Focal adhesion kinase (FAK) is the most essential intracellular integrator in the integrin β1-FAK signalling pathway. Here, we investigated the changes of the two molecules and visualized the possible interaction between them under various hemodynamic conditions in podocytes. Mice kidney tissues were prepared using in vivo cryotechnique (IVCT) and then were stained and observed using light microscopy, confocal laser scanning microscopy and immunoelectron microscopy. The expression of these molecules were examined by western blot. Under the normal condition, integrin β1 stained continuall

SUBMITTER: Yuan X 

PROVIDER: S-EPMC3976409 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature