Hydroxymethylation at gene regulatory regions directs stem/early progenitor cell commitment during erythropoiesis.

ABSTRACT: Hematopoietic stem cell differentiation involves the silencing of self-renewal genes and induction of a specific transcriptional program. Identification of multiple covalent cytosine modifications raises the question of how these derivatized bases influence stem cell commitment. Using a replicative primary human hematopoietic stem/progenitor cell differentiation system, we demonstrate dynamic changes of 5-hydroxymethylcytosine (5-hmC) during stem cell commitment and differentiation to the erythroid lineage. Genomic loci that maintain or gain 5-hmC density throughout erythroid differentiation contain binding sites for erythroid transcription factors and several factors not previously recognized as erythroid-specific factors. The functional importance of 5-hmC was demonstrated by impaired er

SUBMITTER: Madzo J 

PROVIDER: S-EPMC3976649 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature