Specific capture and temperature-mediated release of cells in an aptamer-based microfluidic device.

ABSTRACT: Isolation of cells from heterogeneous mixtures is critically important in both basic cell biology studies and clinical diagnostics. Cell isolation can be realized based on physical properties such as size, density and electrical properties. Alternatively, affinity binding of target cells by surface-immobilized ligands, such as antibodies, can be used to achieve specific cell isolation. Microfluidics technology has recently been used in conjunction with antibody-based affinity isolation methods to capture, purify and isolate cells with higher yield rates, better efficiencies and lower costs. However, a method that allows easy release and collection of live cells from affinity surfaces for subsequent analysis and detection has yet to be developed. This paper presents a microfluidic device th

SUBMITTER: Zhu J 

PROVIDER: S-EPMC3976991 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature