Optimisation of an immunohistochemistry method for the determination of androgen receptor expression levels in circulating tumour cells.

ABSTRACT:

Background

AZD3514 inhibits and down regulates the androgen receptor (AR) and has undergone clinical trials in prostate cancer. To provide proof-of-mechanism (POM) in patients, an immunohistochemistry (IHC) method for determination of AR in circulating tumour cells (CTC) was developed and validated.

Methods

After an assessment of specificity validation focused on intra- and inter-operator reproducibility utilising a novel modification of incurred sample reanalysis (ISR). β-Content γ-confidence tolerance intervals (BCTI) and Cohen's Kappa (κ) were employed in statistical analysis of results.

Results

In a first set of IHC reproducibility experiments, almost perfect agreement was recorded (κ=0.94) when two different operators scored CTC as overall positive or negative fo

SUBMITTER: Cummings J 

PROVIDER: S-EPMC3977890 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature