Computational promoter modeling identifies the modes of transcriptional regulation in hematopoietic stem cells.

ABSTRACT: Extrinsic and intrinsic regulators are responsible for the tight control of hematopoietic stem cells (HSCs), which differentiate into all blood cell lineages. To understand the fundamental basis of HSC biology, we focused on differentially expressed genes (DEGs) in long-term and short-term HSCs, which are closely related in terms of cell development but substantially differ in their stem cell capacity. To analyze the transcriptional regulation of the DEGs identified in the novel transcriptome profiles obtained by our RNA-seq analysis, we developed a computational method to model the linear relationship between gene expression and the features of putative regulatory elements. The transcriptional regulation modes characterized here suggest the importance of transcription factors (TFs) that a

SUBMITTER: Park SJ 

PROVIDER: S-EPMC3977923 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature