An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes.

ABSTRACT:

Introduction

Despite many advances in our understanding of the molecular basis of disc degeneration, there remains a paucity of preclinical models which can be used to study the biochemical and molecular events that drive disc degeneration, and the effects of potential therapeutic interventions. The goal of this study is to characterize global gene expression changes in a disc organ culture system that mimics early nontraumatic disc degeneration.

Methods

To mimic a degenerative insult, rat intervertebral discs were cultured in the presence of TNF-α, IL-1β and serum-limiting conditions. Gene expression analysis was performed using a microarray to identify differential gene expression between experimental and control groups. Differential pattern of gene expression was confirme

SUBMITTER: Markova DZ 

PROVIDER: S-EPMC3978582 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature