Role of HGF in epithelial-stromal cell interactions during progression from benign breast disease to ductal carcinoma in situ.

ABSTRACT:

Introduction

Basal-like and luminal breast cancers have distinct stromal-epithelial interactions, which play a role in progression to invasive cancer. However, little is known about how stromal-epithelial interactions evolve in benign and pre-invasive lesions.

Methods

To study epithelial-stromal interactions in basal-like breast cancer progression, we cocultured reduction mammoplasty fibroblasts with the isogenic MCF10 series of cell lines (representing benign/normal, atypical hyperplasia, and ductal carcinoma in situ). We used gene expression microarrays to identify pathways induced by coculture in premalignant cells (MCF10DCIS) compared with normal and benign cells (MCF10A and MCF10AT1). Relevant pathways were then evaluated in vivo for associations with basal-like subtype

SUBMITTER: Casbas-Hernandez P 

PROVIDER: S-EPMC3978616 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature