Efficiency of whole genome amplification of single circulating tumor cells enriched by CellSearch and sorted by FACS.

ABSTRACT:

Background

Tumor cells in the blood of patients with metastatic carcinomas are associated with poor survival. Knowledge of the cells' genetic make-up can help to guide targeted therapy. We evaluated the efficiency and quality of isolation and amplification of DNA from single circulating tumor cells (CTC).

Methods

The efficiency of the procedure was determined by spiking blood with SKBR-3 cells, enrichment with the CellSearch system, followed by single cell sorting by fluorescence-activated cell sorting (FACS) and whole genome amplification. A selection of single cell DNA from fixed and unfixed SKBR-3 cells was exome sequenced and the DNA quality analyzed. Single CTC from patients with lung cancer were used to demonstrate the potential of single CTC molecular characterization

SUBMITTER: Swennenhuis JF 

PROVIDER: S-EPMC3978840 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature