Runx2 activates PI3K/Akt signaling via mTORC2 regulation in invasive breast cancer cells.

ABSTRACT:

Introduction

The Runt-related transcription factor Runx2 is critical for skeletal development but is also aberrantly expressed in breast cancers, and promotes cell growth and invasion. A de-regulated serine/threonine kinase Akt signaling pathway is implicated in mammary carcinogenesis and cell survival; however, the mechanisms underlying Runx2 role in survival of invasive breast cancer cells are still unclear.

Methods

The phenotypic analysis of Runx2 function in cell survival was performed by gene silencing and flow cytometric analysis in highly invasive MDA-MB-231 and SUM-159-PT mammary epithelial cell lines. The expression analysis of Runx2 and pAkt (serine 473) proteins in metastatic breast cancer specimens was performed by immunohistochemistry. The mRNA and protein level

SUBMITTER: Tandon M 

PROVIDER: S-EPMC3979058 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature