Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

ABSTRACT:

Introduction

The mouse mammary gland provides a powerful model system for studying processes involved in epithelial tissue development. Although markers that enrich for mammary stem cells and progenitors have been identified, our understanding of the mammary developmental hierarchy remains incomplete.

Methods

We used the MMTV promoter linked to the reverse tetracycline transactivator to induce H2BGFP expression in the mouse mammary gland. Mammary epithelial cells (MECs) from virgin mice were sorted by flow cytometry for expression of the mammary stem cell/progenitor markers CD24 and CD29, and H2BGFP. Sorted populations were analyzed for in vivo repopulation ability, expression of mammary lineage markers, and differential gene expression.

Results

The reconstituting act

SUBMITTER: Kaanta AS 

PROVIDER: S-EPMC3979108 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature