Characterization of the miiuy croaker (Miichthys miiuy) transcriptome and development of immune-relevant genes and molecular markers.

ABSTRACT:

Background

The miiuy croaker (Miichthys miiuy) is an important species of marine fish that supports capture fisheries and aquaculture. At present commercial scale aquaculture of this species is limited due to diseases caused by pathogens and parasites which restrict production and limit commercial value. The lack of transcriptomic and genomic information for the miiuy croaker limits the ability of researchers to study the pathogenesis and immune system of this species. In this study we constructed a cDNA library from liver, spleen and kidney which was sequenced using Illumina paired-end sequencing to enable gene discovery and molecular marker development.

Principal findings

In our study, a total of 69,071 unigenes with an average length of 572 bp were obtained. Of these, 45,

SUBMITTER: Che R 

PROVIDER: S-EPMC3979730 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature