Monocyte to macrophage differentiation goes along with modulation of the plasmalogen pattern through transcriptional regulation.

ABSTRACT:

Background

Dysregulation of monocyte-macrophage differentiation is a hallmark of vascular and metabolic diseases and associated with persistent low grade inflammation. Plasmalogens represent ether lipids that play a role in diabesity and previous data show diminished plasmalogen levels in obese subjects. We therefore analyzed transcriptomic and lipidomic changes during monocyte-macrophage differentiation in vitro using a bioinformatic approach.

Methods

Elutriated monocytes from 13 healthy donors were differentiated in vitro to macrophages using rhM-CSF under serum-free conditions. Samples were taken on days 0, 1, 4 and 5 and analyzed for their lipidomic and transcriptomic profiles.

Results

Gene expression analysis showed strong regulation of lipidome-related transcrip

SUBMITTER: Wallner S 

PROVIDER: S-EPMC3979739 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature