Autophagy-dependent production of secreted factors facilitates oncogenic RAS-driven invasion.

ABSTRACT:

Unlabelled

The tumor-promoting functions of autophagy are primarily attributed to its ability to promote cancer cell survival. However, emerging evidence suggests that autophagy plays other roles during tumorigenesis. Here, we uncover that autophagy promotes oncogenic RAS-driven invasion. In epithelial cells transformed with oncogenic RAS, depletion of autophagy-related genes suppresses invasion in three-dimensional culture, decreases cell motility, and reduces pulmonary metastases in vivo. Treatment with conditioned media from autophagy-competent cells rescues the invasive capacity of autophagy-deficient cells, indicating that these cells fail to secrete factors required for RAS-driven invasion. Reduced autophagy diminishes the secretion of the promigratory cytokine interleukin-6

SUBMITTER: Lock R 

PROVIDER: S-EPMC3980002 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature