Measures of gene expression in sputum cells can identify TH2-high and TH2-low subtypes of asthma.

ABSTRACT:

Background

The 3-gene signature of periostin, chloride channel accessory 1 (CLCA1), and Serpin β2 (SERPINB2) in airway epithelial brushings is used to classify asthma into TH2-high and TH2-low endotypes. Little is known about the utility of gene profiling in sputum as a molecular phenotyping method.

Objective

We sought to determine whether gene profiling in sputum cells can identify T(H)2-high and T(H)2-low subtypes of asthma.

Methods

In induced sputum cell pellets from 37 asthmatic patients and 15 healthy control subjects, PCR was used to profile gene expression of the epithelial cell signature of IL-13 activation (periostin, CLCA1, and SERPINB2), TH2 genes (IL4, IL5, and IL13), and other genes associated with airway TH2 inflammation.

Results

Gene expression l

SUBMITTER: Peters MC 

PROVIDER: S-EPMC3981552 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature