Automated quantification reveals hyperglycemia inhibits endothelial angiogenic function.

ABSTRACT:

Objective

Diabetes Mellitus (DM) has reached epidemic levels globally. A contributing factor to the development of DM is high blood glucose (hyperglycemia). One complication associated with DM is a decreased angiogenesis. The Matrigel tube formation assay (TFA) is the most widely utilized in vitro assay designed to assess angiogenic factors and conditions. In spite of the widespread use of Matrigel TFAs, quantification is labor-intensive and subjective, often limiting experiential design and interpretation of results. This study describes the development and validation of an open source software tool for high throughput, morphometric analysis of TFA images and the validation of an in vitro hyperglycemic model of DM.

Approach and results

Endothelial cells mimic angiogenesis w

SUBMITTER: Prisco AR 

PROVIDER: S-EPMC3981811 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature