D-Tailor: automated analysis and design of DNA sequences.

ABSTRACT:

Motivation

Current advances in DNA synthesis, cloning and sequencing technologies afford high-throughput implementation of artificial sequences into living cells. However, flexible computational tools for multi-objective sequence design are lacking, limiting the potential of these technologies.

Results

We developed DNA-Tailor (D-Tailor), a fully extendable software framework, for property-based design of synthetic DNA sequences. D-Tailor permits the seamless integration of multiple sequence analysis tools into a generic Monte Carlo simulation that evolves sequences toward any combination of rationally defined properties. As proof of principle, we show that D-Tailor is capable of designing sequence libraries comprising all possible combinations among three different sequence

SUBMITTER: Guimaraes JC 

PROVIDER: S-EPMC3982154 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature